Risks of Insertional Mutagenesis by DNA Transposons in Cancer Gene Therapy

Posted on by immusoft

Recently the Sleeping Beauty (SB) transposon/transposase system has been applied in clinical trials to redirect T-cell specificity through the addition of a transgenic cassette that drives expression of a chimeric antigen receptor (CAR). We have focused on issues relating to insertional mutagenesis in the context of the plasticity of human genomes, the unexpected variability in human genomes elucidated by recent high-throughput, wholegenome sequencing projects of hundreds of individuals and cells therein, and the recently discovered high rates of remobilization of endogenous transposable elements…